Enumeration of megakaryocytes number in thrombocytopenic patients by immunohistochemistry

Doaa I. Elzaeem, Esmat A El Sharkawe, Ayman G Ghobrial, Waleed M Abd El-Hamed, Aliaa S Abd El Fatah

Abstract


Background and objectives: The aim of the present study is to enumerate megakaryocytes in vivo in patients with thrombocytopenia.

 Subjects and methods: The study was carried out on 20 ITP (group I) patients and 20 thrombocytopenic patients with myelodysplastic syndrome (groupII) who were admitted to minia university hospital . Serial histological sections from bone marrow biopsies were stained for CD61. The number of bone marrow megakaryocytes were determined morphologically .Results: Megakaryocytes number was increased in ITP and MDS patients (group I & II ) and there was no statistically  significant difference  between both groups  (Mean ± SD 8.1 ±5.1 vs. 6 ±3.1 cells per HPF; p=0.271). Conclusion: researches in ITP should focus on ultrastructure of megakaryocytes and its relation to antibody.


Keywords


ITP, megakaryocytes, antibody specificity

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