Increased transcriptional expression of Epstein-Barr virus (EBV) BamHI-A Rightward Transcripts: RPMS, BARF0 and A73 in EBV-associated nasopharyngeal carcinoma patients

Thuan Duc Lao, Tuan Hoang Anh Nguyen, Dung Huu Nguyen, Thuy Huyen Ai Le

Abstract


Background: In Vietnam, nasopharyngeal carcinoma (NPC) is the most common cancer of head and neck cancers, with a high rate of cancer deaths. Epstein-Barr virus (EBV) infection has been reported to be an etiological factor of NPC. The transcripts from the Bam A rightward transcripts (BamHI-A) region, including BARF0, RPMS1 and A73, which mapped to EBV genome, have been indicated to play a pivotal role in EBV biological stage. However, the profile of target genes in Vietnamese NPC patients has not been investigated.

Purpose: The aims of current study were to evaluate the expression and assess whether or not BARF0, RPMS1 and A73 transcripts associated to Vietnamese NPC.

Materials and methods: Total of RNA was isolated from 30 NPC biopsy and 30 noncancerous swab samples were analyzed by RT-qPCR. The 2-ΔΔCt method was applied to evaluate the relative expression of each target gene by q-PCR.

Results: The higher frequency of BARF0, RPMS1 and A73 detection were 66.67%, 90.00%, and 70.00%, respectively, in NPC samples (p < 0.05). Additionally, the BARF0, RPMS1 and A73 transcripts were overexpressed by 9.58, 10.22 and 20.58 times in NPC samples compared to noncancerous samples. Additionally, the highest OR and RR values were 29.00 (95% CI = 3.49 – 241.14), and 2.75 (95% CI = 1.79 – 4.23), respectively, observed in the case of combination of detected three genes compared to individual genes.

Conclusion: Taking together, it could be concluded that BARF0, RPMS1 and A73 transcripts were upregulated in NPC, and maybe further serve as potential diagnostic biomarkers for NPC in local population. 


Keywords


BARTs; BARF0; RPMS1; A73; Nasopharyngeal carcinoma

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